GC-AutoFit Calibration
Calibration Dashboard
Calibration Equation Library Generation
Review job status, download the generated library files, and inspect the resulting table output from this calibration run.
This page reflects the submitted pipeline, internal standard, uploaded spectra package, and generated supplementary outputs.
StatusCompleted
Outputcalibration_equations.csv
Internal StandardTropic acid (ISTD)
BiofluidUrine
Samples11
Runtime2.09 minutes 5.53 seconds
Run Details
Submitted configuration and generation settings for this calibration job.
Library PipelineCalibration Equation Library Generation
Internal StandardTropic acid (ISTD)
BiofluidUrine
Blank ProvidedYes
Number of Samples11
MF Score Cut Off400.0
DateJuly 25, 2025 13:52
Runtime2.09 minutes 5.53 seconds
Status
Monitor processing, review errors, or download the generated calibration outputs when the job is complete.
Library Generation Complete
Your calibration output files are ready to download. Use the calibration_equations.csv for the next workflow step and the Peak Areas and Ratios for review and troubleshooting.
Generated Table
Review the exported table directly in the browser before downloading the final CSV output.
| Compound | CompoundWithTMS | avg_Target_EIC_RT_min | Avg_RI | a||b||c | Intercept(High||Low) | Slope(High||Low) | RSquared | RSquaredAdj | Peak Area Ratios | Theoretical Concentrations | Concentration_threshold(uM) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2-Hydroxyisovaleric Acid | 2-Hydroxyisovaleric Acid | 12.75 | 1179.4 | NA||NA||NA | NA||0.04 | NA||14.16 | 0.98 | 0.98 | 0.021591982208446558 0.07322640744410941 0.28065774405883503 1.404124154064441 3.7342866899618845 5.385371105160023 | 0.003 0.01 0.03 0.089 0.222 0.4 | |
| 2-oxo-3-methylvaleric acid | 2-oxo-3-methylvaleric acid - Peak1 | 13.17 | 1192.29 | NA||NA||NA | NA||0.03 | NA||0.16 | 0.59 | 0.53 | 0.0006373994525270387 0.003239674980479473 0.007403574447779825 0.04439116193711983 0.11655415870553698 0.08259721621030819 0.20261569978368799 0.21482166066417843 0.0939316139925569 | 0.003 0.01 0.03 0.089 0.222 0.4 0.641 0.833 1.0 | |
| Maleic | Maleic | 16.44 | 1316.7 | NA||NA||NA | NA||0.01 | NA||2.2 | 1.0 | 1.0 | 0.0033849456091800665 0.01053465962814066 0.0702002171284686 0.41665950990118106 1.1354415883160707 1.8976628975718846 2.639899280866531 3.755287746134048 4.381760262583272 | 0.007 0.02 0.059 0.178 0.444 0.8 1.282 1.667 2.0 | |
| Succinic acid | Succinic acid | 16.64 | 1325.81 | NA||NA||NA | NA||-0.01 | NA||2.56 | 1.0 | 1.0 | 0.0029405250346891854 0.009522158085493096 0.06229397643058871 0.3548484088902176 0.9365332669382053 1.527406368911888 2.287701490785093 3.353238339784091 3.7862269022636372 | 0.005 0.015 0.044 0.133 0.333 0.6 0.962 1.25 1.5 | |
| 2-Hydroxybenzoic acid(salicylic acid) | 2-Hydroxybenzoic acid(salicylic acid) (1TMS) | 17.31 | 1355.09 | NA||NA||NA | NA||-0.0 | NA||0.01 | 0.93 | 0.9 | 1.650730042775648e-05 0.0017256452064954412 0.006028536453278752 0.007367217540011097 | 0.005 0.311 0.56 0.897 | |
| 5-hydroxyhexanoic | 5-hydroxyhexanoic 2tms | 17.65 | 1369.52 | NA||NA||NA | NA||0.27 | NA||0.52 | 0.24 | 0.13 | 0.011294547165447399 0.03865477838963201 0.1090484437042465 0.37431934065363626 0.830667257920206 0.6197003655583833 1.0522564713732816 0.923707633811386 0.18047213954522276 | 0.003 0.01 0.03 0.089 0.222 0.4 0.641 0.833 1.0 | |
| 2-Hydroxybenzoic acid(salicylic acid) | 2-Hydroxybenzoic acid(salicylic acid) (2TMS) | 20.97 | 1522.09 | NA||NA||NA | NA||0.54 | NA||4.81 | 0.86 | 0.83 | 0.030632394447289706 0.11212324603598897 0.3507482105159055 2.0445339253650117 3.0160187837470014 3.196637519494433 4.451455591458785 | 0.005 0.014 0.041 0.124 0.311 0.56 0.897 | |
| O-Hydroxyphenylacetic acid | O-Hydroxyphenylacetic acid | 22.15 | 1580.72 | NA||NA||NA | NA||0.15 | NA||3.0 | 0.99 | 0.99 | 0.016134855912465516 0.059161416431307894 0.17051423044823727 0.7019983441522116 1.787849148227935 2.852404759128181 3.9748544797030108 5.363122372331292 5.825535815970862 | 0.007 0.02 0.059 0.178 0.444 0.8 1.282 1.667 2.0 | |
| p-Hydroxyphenylacetic acid | p-Hydroxyphenylacetic acid 2TMS | 23.48 | 1651.12 | NA||NA||NA | NA||0.63 | NA||3.69 | 0.91 | 0.9 | 0.027631436343045133 0.1025019063664932 0.30550183894080846 1.2476203347606298 3.070551095710991 4.952047551505992 6.359282984390488 6.661389918620095 6.789316038433737 | 0.007 0.02 0.059 0.178 0.444 0.8 1.282 1.667 2.0 | |
| trans-Aconitic acid | trans-Aconitic acid (3TMS) | 25.65 | 1771.12 | NA||NA||NA | NA||0.49 | NA||2.29 | 0.46 | 0.39 | 0.0016890596797476437 0.01490697200672246 0.06041545452955217 0.7075306993625025 2.4313077796137494 4.163200108849518 5.2013636637875456 0.08390006803158555 6.563890744811105 | 0.007 0.02 0.059 0.178 0.444 0.8 1.282 1.667 2.0 | |
| Homovanillic acid | Homovanillic acid 2TMS | 25.93 | 1787.51 | NA||NA||NA | NA||0.08 | NA||6.35 | 0.99 | 0.99 | 0.010164376040832227 0.04360293185555232 0.14396435450375553 0.6428992152696132 1.6909278359566393 2.9241836552370026 3.9959244446568003 5.563942579682729 6.2142259723278235 | 0.003 0.01 0.03 0.089 0.222 0.4 0.641 0.833 1.0 | |
| 1H-Indole-3-acetic acid | 1H-Indole-3-acetic acid (2TMS) | 29.09 | 1977.12 | NA||NA||NA | NA||0.08 | NA||0.11 | 0.22 | 0.11 | 0.004139608529563771 0.01544213524569137 0.06518025453833935 0.2579406588335326 0.1341484705731138 0.0571802863456559 0.128925749184608 0.18862247481780048 0.17298471280428152 | 0.003 0.01 0.03 0.089 0.222 0.4 0.641 0.833 1.0 | |
| 2-oxo-3-methylvaleric acid | 2-oxo-3-methylvaleric acid - Peak2 | 13.78 | 1214.37 | NA||NA||NA | NA||0.0 | NA||0.25 | 0.81 | 0.78 | 0.00079833708318383 0.0024778115040105606 0.01772788334898834 0.05619392909095625 0.1013643362542443 0.19362739555079486 0.297864646995705 0.16970124458755342 | 0.01 0.03 0.089 0.222 0.4 0.641 0.833 1.0 | |
| Citric Acid | Citric Acid 4TMS | 26.98 | 1849.55 | NA||NA||NA | NA||0.02 | NA||0.85 | 0.68 | 0.63 | 0.0040925197311912865 0.021294412928340868 0.16119030100788667 0.5338784744855691 1.1093987015315117 0.6210806387775215 0.742401566372279 2.388230860715679 | 0.02 0.059 0.178 0.444 0.8 1.282 1.667 2.0 | |
| 1H-Indole-3-acetic acid | 1H-Indole-3-acetic acid (1TMS) | 28.22 | 1923.62 | NA||NA||NA | NA||0.05 | NA||1.52 | 0.96 | 0.96 | 0.005223229029616981 0.06710742274250539 0.57550830000216 0.6897250282627194 1.0359566070468398 1.3633674857193976 1.4664887417142067 | 0.03 0.089 0.222 0.4 0.641 0.833 1.0 |